1. Make sure you have your image on its own layer with no background.

This is the #1 mistake people make, in older versions of Photoshop you would have saved it on a colored background

2. Now go File>Save for web

You should see this box open.

3.Make your setting gif and use the smallest amount of colors you can without affecting the appearance of the image.

The 2 things to remember here are:

1. Make sure Transparency is selected.
2. Set the Matte color to the closest match of the background color you will be placing the image on. If you are unsure then set the Matte to "None" (more about Matte in step 4)

Click ok and save your image.

4. Some more information on Matte.

Matte puts a thin outline around the image to get rid of what we call halos.

On the right you can see an example of a halo. Look familiar? This is the sign of an amateurish webpage. Believe me there are already enough bad webpages out there without us adding to them. :)

5. Here is the same image with Matte set to none. Notice the halo is gone, but it is still a bit jaggy around the edges.

6. Here is the same image, this time saved with a black Matte. Notice how nice and sharp it is?

Always choose a matt color if you can. This way all your transparent gif's will look nice and clean.

This Matt trick will also work with transparent PNG's.

Stay tuned for more :)